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| Topic | Description |
| Mobile Phase | Understanding the role and selection of the liquid mobile phase in chromatographic separations. |
| Stationary Phase | Explaining the importance and characteristics of the stationary phase in liquid chromatography. |
| Detector | Learning about different detectors used to measure analytes separated by liquid chromatography. |
| Data Acquisition and Processing | Understanding the methods and techniques for data acquisition and processing in LC analysis. |
| Quantitative Analysis | Explaining how liquid chromatography is used for quantitative determination of analytes. |
| Qualitative Analysis | Discussing the application of liquid chromatography for qualitative identification of compounds. |
| Liquid Chromatography-Mass Spectrometry | Introduction to the hyphenated technique LC-MS and its applications in chemical analysis. |
| Achievable Separations | Understanding the factors affecting the separation efficiency and resolution in liquid chromatography. |
